Proteomics & Protein Analysis Calculators

Proteomics & Protein Analysis Calculators

Proteomics and protein analysis calculators simplify interpretation of peptide and protein data for researchers, students, and bioinformaticians. These interactive tools estimate peptide mass, charge-to-mass ratios, digestion patterns, and basic physicochemical properties critical in MS-based proteomics and protein characterization.

Instantly compute theoretical peptide masses, estimate isoelectric point (pI), GRAVY hydrophobicity, trypsin cleavage counts, or protein coverage percentages. Each calculator applies standard biochemical equations and simplified assumptions used in peptide mapping, quantitation, and bioanalytical workflows.

⚠ Disclaimer: These calculators provide simplified estimates for educational and research purposes only and are not intended for clinical diagnostics or regulatory use.

Peptide Mass Calculator

Sum of residue masses (monoisotopic, no modifications)

Isoelectric Point (pI) Estimator

pI ≈ 7 + 0.5 × (basic − acidic residues)

Hydrophobicity (GRAVY) Calculator

GRAVY = mean hydropathy value

Trypsin Digestion Peptide Count

Number of peptides = cleavage sites + 1

Protease Cleavage Site Counter

Counts residues matching protease specificity

Mass Spec m/z Calculator

m/z = (mass + z × 1.0073) / z

Protein Coverage Estimator

Coverage = (Σ peptide aa / total aa) × 100%

Label-Free Quantitation (LFQ) Fold Change

Fold-change = B / A

Isoform Abundance Estimator

Abundance = (isoform peptides / total) × 100%

Phosphorylation Stoichiometry

Stoichiometry = (modified / total) × 100%